Runes are permanent upgrades which enable the player to travel to locations and Biomes which are not accessible otherwise.
Once acquired, the player's collected runes can be viewed in the saved game slot (in the main game menu, under "play")
The only way to acquire runes is to travel to specific zones and defeat Elite enemies which spawn from certain Elite obelisks.
There are currently six runes in Dead Cells: the Vine Rune, the Teleportation Rune, the Challenger Rune, the Ram Rune, the Spider Rune, and the Homunculus Rune.
|Rune||Image||Location - Zone||Location - Environment||Elite Guardian or Boss||Unlocks - Objects||Unlocks - Zones|
|Vine Rune||Promenade of the Condemned||In a room accessed by entering a door found at the base of a large overhang||Undead Archer||Ability to sprout vines from special green blobs.||Toxic Sewers, Ramparts|
|Teleportation Rune||Toxic Sewers||In a room found beneath a switch-operated door with the switch directly behind the door.||Slasher||Ability to teleport between pairs of electric coffins.||Ossuary, Forgotten Sepulcher|
|Challenger Rune||The Black Bridge||Defeat The Concierge||The Concierge||Opens the daily run door in The Prisoners' Cells.||Daily Run|
|Ram Rune (formerly known as "Belier's Rune")||Ossuary||In a room in Ossuary, in which the buttons that open the door in question are beneath fragile ground.||Slasher||Ability to break fragile ground (marked with yellow sign) with any dive attack.
Buffs up your Dive attack's damage and range (long fall Dive attack only).
|Spider Rune||Slumbering Sanctuary||In a room found somewhere in Slumbering Sanctuary that requires wall-jump to get out.||Caster||Ability to climb the walls and wall-jumping.||Slumbering Sanctuary (if entered from Black Bridge), Graveyard, The Prison Depths|
|Homunculus Rune||Prisoners' Quarters||Defeat The Hand of the King.||The Hand of the King||Allows the player to control their 'head' separate from their body to access different areas of each level and disable a single enemy's movements with full control of the player's body.
Upon reaching max distance away from the main body the head will slingshot back to position.
The cooldown for this rune will not start unless the player latches onto an enemy.
This can also be used indefinitely to bring blueprints and loot back to the player.
Homunculus Rune does not break invisibility.