Runes are permanent upgrades which enable the player to travel to locations and Biomes which are not accessible otherwise.
The only way to acquire runes is to travel to specific zones and defeat Elite enemies which spawn from certain Elite obelisks. Once acquired, the player's collected runes can be viewed on the right side of the pause screen at any time.
There are currently five runes in Dead Cells: the Vine Rune, the Teleportation Rune, the Challenger Rune, Belier's Rune, and the Spider Rune.
|Rune||Image||Location - Zone||Location - Environment||Elite Guardian||Unlocks - Objects||Unlocks - Zones|
|Vine Rune||Promenade of the Condemned||In a room accessed by entering a door found at the base of a large overhang||Undead Archer||Ability to sprout vines from special green blobs.||Toxic Sewers|
|Teleportation Rune||Toxic Sewers||In a room found beneath a switch-operated door with the switch directly behind the door.||Slasher||Ability to teleport between pairs of electric coffins.||Ossuary, Forgotten Sepulchre|
|Challenger Rune||The Black Bridge||Defeat the Incomplete One.||The Concierge||Opens the daily run door in The Prisoners' Cells.||Daily Run|
|Ram Rune (formerly known as "Belier's Rune")||Ossuary||In a room in Ossuary, in which the buttons that open the door in question are beneath fragile ground.||Slasher||Ability to break fragile ground (marked with yellow sign) with any dive attack.
Buffs up your Dive attack's damage and range (long fall Dive attack only).
|Spider Rune||Slumbering Sanctuary||In a room found somewhere in Slumbering Sanctuary that requires wall-jump to get out.||Orb Caster||Ability to climb the walls and wall-jumping.||From The Black Bridge to Slumbering Sanctuary, Graveyard, The Prison Depths|